You can extract toxin using sonicator,however it needs standardization

 Dear Shashikant Sir, I want to know about the procedure for endotoxins specially. Is there any kit available in the market for endotoxin extraction?

Dear Istakar

It's very hard work but you can read this paper entitled 

"Insecticidal Toxin Complex Proteins from

Xenorhabdus nematophilus: Structure and Pore Formation"

 by Joel J. Sheets et al., 2011. It'll help you. 

Hanan

Dear Hanan Ma'am, I want to know about the Endotoxins extraction procedure.

Dear sir,  

I have a query that, Do these nematode symbiotic bacteria produces endotoxin majorly ??  As these have produces the exotoxins complexes viz., protease, metaloproteases, chitinase etc. which are responsible for higher and quick knockdown of insect.

Dear Ramesh Aravind,

Endotoxins are lipopolysaccharides (LPS) and they are present in bacterial cell wall. Generally, they released when bacteria died. They are also found to suppressive the host's immunity. And the majority of produced toxins without no doubt are exotoxins.

Dear Istkhar

I understand your question so I recommended that paper because Joel Sheets works on the endotoxins comes from the cells. I still work on this approach. any way this is the methods

 Cell pellets had been obtained from a 2 liter culture of Xenorhabdus or Photorhabdus spp. fermentation for 24h. The pellets had been suspended in 50 mM Tris-HCl pH 8.0, 100 mM NaCl, 1mM DTT, 10% glycerol and lysozyme (0.6 mg/ml). A small amount of glass beads (0.5 mm dia.), had been added and cells had been disrupted by sonication. Then broken cells had been centrifuged at 10,000 rpm for 60 min. at 4°C., supernatant was then collected. A bacterial protease inhibitor cocktail had been added (Sigma, St. Louis), and the solution had been dialyzed against 25 mM Tris-HCl, pH 8.0 overnight. The protein was then loaded onto a Q Sepharose XL (1.6 x 10 cm) anion exchange column, (Pharmacia). Bound proteins were eluted using a linear 0 to 1 M NaCl gradient in 5 column volumes. The high molecular weight toxin complexes were eluted in the early fractions. Bound and unbound fractions were obtained.

He used four columns to get purified toxin. Just read the paper.

There are another kind of toxin in the outer cell membrane of the bacteria. 

Hope these information can help you.

Hanan