Would appreciate help,
I try to extract yeast DNA, I have a very small amount of yeast
And among them a detergent because they have been treated
I want to extract plasmid DNA from them for PCR applications
I tried putting Zimolase then to transfer all for PCR and didn't work
Will yeast boiling break their cell wall?
I thought to boil the yeast and then to do PCR CLEAN UP and to be left only with the DNA
And then proceed to PCR. Is it possible?
thank you
May
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