May Meltzer

4 Questions 10 Answers 0 Followers

Questions related from May Meltzer

How to extract DNA out of the yeast?

Would appreciate help, I try to extract yeast DNA, I have a very small amount of yeast And among them a detergent because they have been treated I want to extract plasmid DNA from them for PCR...

01 July 2020 6,707 0 View

How do you choose the detergent concentration for protein purification?

hey i am using 2%OG and 1%DDM what concentration do i need to use with DM? is there a formula to calculate? Why usualy using 1%DDM and 2% OG (what % is higher then the CMC?) thanks

01 January 2019 4,105 8 View

How can i produce genomic analysis (DNA extract) with very low concentration of DNA from Yeast?

hey all i need to extract DNA from yeast cells. but how can i do it from one yeast or very low number of yeast cells. is there a special protocol for it? Thanks May

18 June 2017 5,979 9 View

Hey i am looking for a way to make pores in the cell wall of a yeast without destroying it completely. i have tried zymolase and OG, any suggestions?

its an yeast cell wall looking for a way to make pores in it but not to destroy it completely

22 January 2017 6,070 10 View