How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Why are the modelled and circuit system different in response for controlled buck converter?

[Images are attached at bottom] I am currently working on a buck converter controlled by two different control methods, Reinforcement learning applied to find PID values & a lead-lag...

16 February 2021 5,060 10 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Why do some full text requests not allow me to respond to the requesting person?

Over the last year, when I have clicked on 'View the Request' in the notifying e-mail for a full text request, I have been taken to the paper in question but there is no request visible and thus I...

16 January 2021 7,558 1 View

Spectrabase no longer has overlay function?

Hello, I am a quality control chemist and I test raw materials for a nutritional supplement company. I make heavy use of FTIR-ATR for identification. We are a small laboratory with a limited...

07 January 2021 8,850 2 View

Follow up tests to explore interaction effects in a multiple logistic regression: can someone help explain my contradictory results?

I am conducting a logistic regression in order to examine whether there is an interaction between the number of stressors participants were currently experiencing (between 0-9) and their current...

20 December 2020 1,043 9 View

How to stain adherent cells with DAPI?

Hello! I am growing transfected cells in 24 well plate. on the bottom of each well i have a small glass cover-slip. so the cells adhere to that cover slip. I am using this method because its very...

15 December 2020 7,121 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Help to set up an in vitro Bioaccessibility assay?

Hi everyone! i am looking for protocols to evaluate the bioaccessibility of carotenoids in plant matrix. Ideally the best would be a quite easy protocol that could be used massively in the lab,...

02 February 2021 5,516 2 View

Equation to calculate Chlorophylls and carotenoids concentrations from a MeOH:HCl 1% (v/v) plant extract solution?

Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....

25 January 2021 3,686 2 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

Edu assay by spectrophotometry means?

Dear all, Have any one performed edu assay by spectrophotometric means ? I'm looking for a kit for that purpose. I have Chinese kit for this purpose, I followed their protocol but the result is...

24 November 2020 1,766 3 View

Bisphenol A in water - measuring bpa protocol using a uv/vis spectrophotometer?

Hi, I'm interested in a protocol for measuring concentration of BPA in distilled water (no interference) using a uv/vis spectrophotometer . The method found...

09 November 2020 7,012 3 View

What blank should be used to measure protein and nucleic acid content within conditioned medium by spectrophotometry ?

I want to measure protein and nucleic acid within conditioned medium. I used nanodrop with fresh medium as blank. Yet, I got a weird result, the blank absorbance is higher than the sample....

11 October 2020 3,139 3 View

What are the simple protocols of preparing plant sample for GCMS Analysis and UV viz spectrophotometry?

To study and analyze plant extract through GCMS and UV viz spectrophotometry, i want protocols that how can I prepare sample for both technique.

19 September 2020 821 3 View

Is there any cost-effective, simple and efficient way to analyse and estimate the amount of vitamins in algal samples?

I am currently pursuing my research in Phycology. I'd like to know if there's any simple and cost-effective method to detect and analyze nutrients and vitamins in the algal matter. The experiment...

18 September 2020 4,081 3 View