Hi,
I want to extract amino acids and nucleosides from samples with high salt/buffer, protein, fat and cellular debris. Amino acids and nucleosides will then be analyzed by LC-MS.
Do you know how I can isolate amino acids from my sample? And how I can isolate the nucleosides? (two different analyses).
We are modelling the digestive process so there is digested food stuff (blended before mixing with digestive enzymes) in the substrate that varies depending on the experiment and the substrate used.
The digested substrate is in 40 mL of liquid.
I would like to avoid MWCO filtration if possible.
Thanks
Hi Florine,
There are many methods available to chose from in the litterature, so you can pick what suits your samples/time/available instrumentation or other considerations.
E.g. nucleosides can be extracted in alkaline methanol : Article Simultaneous Quantification of Nucleosides and Nucleotides f...
free amino acids can be extracted with 4 volumes methanol or similar extraction solvents.
It sounds like you have a matrix that is at least partly comprised of known components, in which case it may be possible to test the optimal methods by spike-in of your compounds of interest.
I suggest you search for litterature on the relevant methods, it should not be hard to find examples.
https://doi.org/10.3389/fbioe.2020.552335
https://doi.org/10.2144/000112401
https://www.intechopen.com/chapters/49705
https://www.intechopen.com/chapters/40716
https://experiments.springernature.com/articles/10.1385/0-89603-250-7:189
https://www.nextadvance.com/homogenization/nucleotide-extraction/
Hi, u can check this link its will help
http://dx.doi.org/10.1007/s13361-019-02140-7
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