What type of fusion are you doing? People frequently use maltose-binding protein as N-terminal fusion partner (assuming the N-terminus of your receptor is extracellular) - of course you need to make sure your constructs contains the appropriate signal sequence for your expression system. As C-terminal (intracellular) fusion partner, sfGFP may be useful, as it gives you immediate feedback on expression level and sub cellular localisation of your protein. Often several different receptor truncations, linker lengths and fusion partners have to be tried to find the combination that's best for your protein.

What type of fusion are you doing? People frequently use maltose-binding protein as N-terminal fusion partner (assuming the N-terminus of your receptor is extracellular) - of course you need to make sure your constructs contains the appropriate signal sequence for your expression system. As C-terminal (intracellular) fusion partner, sfGFP may be useful, as it gives you immediate feedback on expression level and sub cellular localisation of your protein. Often several different receptor truncations, linker lengths and fusion partners have to be tried to find the combination that's best for your protein.

How are you detecting expression of this fusion protein (or lack thereof :-)? Also, is this a construct targeted for the secretion pathway, or just cytoplasmic?

Hi Alejandro

The construct is just an expression vector, however, the protein of interest has a leader sequence at the N-terminus, thereby allowing for its appropriate targeting at the membrane of the cells. I have been using western blots and immunofluorescence to as my read outs for expression.