Hi 

I am trying to express a fusion protein where one of the protein is a receptor protein and the other is a soluble protein. I have cloned them together but for some reason, I have not been able to express the fusion protein. I have tried making lentivirus and transduced mammalian cell line and I have had no luck with that either. Could someone provide their insights into constructing fusion proteins, using linkers, using tags and how to design these things with respect to solubility and flexible structure that it does not affect the normal folding of the 2 proteins in the fusion construct? 

Thanks

Similar questions and discussions