Kaushal Baid

5 Questions 5 Answers 0 Followers

Questions related from Kaushal Baid

Has anyone run dsRNA immunoblots?

Hi everyone I am trying to run an immunoblot for dsRNA molecules of specific lengths. I have been running 5% PAGE gels and transferring the RNA using semi-dry transfer @ less than 10V. Note: the...

08 August 2016 2,925 1 View

Does anybody know a good lot of the Santa Cruz IRF3 FL-425 antibody?

I am looking for a good lot of the Santa Cruz IRF3 FL-425 antibody (sc-9082). My lab had purchased that anybody a couple years ago and received a great lot. When we ran out of that antibody and...

06 June 2016 6,299 0 View

Ways around expressing a protein which is seemingly toxic for the cells at higher concentrations in mammalian cancer cell line?

Hi everyone Background: Expressing a membrane protein in A549 cells (lung epithelial cancer cell line) using a expression vector under the constitutive CMV promoter. I am able to detect the...

03 March 2016 400 4 View

How to express a fusion protein where one of the protein is a receptor protein?

Hi I am trying to express a fusion protein where one of the protein is a receptor protein and the other is a soluble protein. I have cloned them together but for some reason, I have not been able...

01 January 2016 5,052 4 View

Is there a specific gene sequence that can be used between 2 proteins which are to be fused but can be inducibly cleaved in mammalian cells?

Hi I am trying to fuse 2 proteins and express them in mammalian cells. One of the proteins is a membrane receptor and the other is a soluble protein. I have to create 2 stable cell lines, one...

01 January 2016 797 2 View