I intent to study the subcellular localization of a protein. I am going to produce the expression construct by fusing a GFP gene sequence to the 3' end of a gene, so that the fusion protein will contain the GFP at the c-terminal of my protein of interest.
Should i provide some spaces between my protein of interest and the GFP by introducing some additional nucleotide sequence in the construct? if yes, what is the recommended sequence to be added?
Or i can fuse the two gene sequences without any gap (without additional nucleotides).
Hopefully there are some experts willing to help me with that. Thank you in advance.
Yusuf Chong Yu Lok Make use of a vector and insert your gene in front of the GFP gene. Your gene's stop codon should be eliminated, and your gene and the GFP gene should be in the frame.
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