Can I get identification keys for identifying mermaid purses ?

01 January 1970 2,100 2 View

How can i interpret K- dominance curve created using PRIMER?

Dear friends, I had created a k-dominance curve from species abundance data of monthly samples from an ecosystem, i am ambiguous about the interpretation and explanation for the same, the curve...

01 January 1970 4,399 1 View

What kinds of transformation ins required to analyze diversity indices using PRIMER ?

01 January 1970 4,625 1 View

What are the methodologies used for analyzing the correlations between diversity and the environment ?

01 January 1970 6,655 2 View

How can identify hermaphroditism in teleost fishes?

01 January 1970 8,686 1 View

How to present a sample based rarefaction curve?

01 January 1970 2,877 2 View

Is it possible to plot Species Distribution Models using "R" Programme ?

01 January 1970 1,458 2 View

What is the difference between ANOSIM and ANOVA?

Dear friends, Can any one help me to sort out the difference between ANOSIM and ANOVA? Many thanks in advance Sileesh Mullasseri

01 January 1970 9,456 3 View

R packages for biotechnology and bioinformatics?

Dear friends, Now i am exploring R packages for biotechnology and bioinformatics including big data analysis for my project. kindly help me to suggest which package is widely...

01 January 1970 8,362 5 View

How to explain MDS plot ?

Dear friends, I am trying to use MDS for linking environment data with abundance, abundance added as vector for analyzing correlation with environment data , Pearson's...

01 January 1970 3,100 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View