I am looking for the protocol(s) to evaluate the antifungal properties of some PGPRs against soil-borne plant pathogens in lab conditions. Kindly guide me. Thank you.
Inam-ul-Haq et al., 2003). Direct and indirect confrontation between the expected PGPR and the pathogen fungi. Greetings
You'll need good aseptic technique, laminar flow hood, autoclave, etc. In short, apply fungal pathogen onto Petrie dish with appropriate media (PDA?). Spot plate with solution of known concentration of PGPR extract. Incubate. Look for zones of inhibition - location of spot and beyond showing no pathogen growth. Active extracts may be fractionated and assayed to ultimately ID a single active compound.
Hi Muhammad,
There are several methods to test antifungal activity of bacteria (or PGPRs) against soil-borne (bacterial/fungal) plant pathogens in vitro using petri plates.
The most common one is called "Dual-culture assay, also known as co-culture". It means that you will co-culture your bacteria with a pathogen in a same petri plate to see antagonistic actions (PDA or solid medium required).
Another one can be "microtiter broth dilution method", which requires culture broth of your PGPRs. Using this method, you can not only determine the inhibitory activity of your PGPRs, but also the minimum inhibitory concentration (MIC) of your culture broth.
Both of these methods are thoroughly described in our Biocontrol paper, which is available at Article Identification, Characterization, and Efficacy Evaluation of...
Good luck,
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