How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

How do I avoid sucking up electrospun scaffolds during cell culture?

Hi, I'm a PhD student working to develop electoconductive, electrospun scaffolds for cardiac tissue engineering applications. I have been seeding cells onto these scaffolds but having major...

04 December 2020 6,672 3 View

Can I autoclave d-mannose?

Hello everyone, I was wondering whether I should autoclave or filter d-mannose solution. In our laboratory, we autoclave glucose separately when we make YPD media for yeast culture. So, I am...

08 October 2020 7,068 1 View

Does topology from ATB support FF other than Gromos?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

07 June 2020 8,989 2 View

How can I use a molecule from ATB in Gromacs?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

06 June 2020 2,112 4 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

Does anyone know about analysis of 3D mapping and depth profile for wooden object?

I have 1cm cubic size wood samples. inside of wood, there are S and S-organic, inorganic compounds randomly. I'd like to figure out the quantities of S distribution non-destructively. First, I...

20 February 2020 9,303 2 View

Is there any atmosphere that Titanium chloride can make fume?

I use TiCl4 to check if there's water in the glovebox. when I opened TiCl4 there were a lot of fumes, but I never guess where the water comes. Because there was no oxygen inside. So I didn't sure...

13 January 2020 9,127 1 View

Is it viable to replace ray-traced 3d simulation visualization with Neural network (say GAN)?

My problem may not be very general. Imagine you observe the sun through thick clouds in multiple wavelengths. Given the spectrum of light starting off the sun's surface, you can calculate how rays...

08 January 2020 4,764 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View