I am using Peakfit 4.12 for FTIR deconvolution, and apply the second derivative method, but I can't confirm the number of bands in a certain wavenumber region. Is there any useful way to conduct this step?
2nd derivative is the normal way to perform the operation. I am not sure what it is not possible to confirm the number of bands.
Reduce your wavenumber region to visualise them better?
Perform a smoothing before differentiation?
The best way to know exactly how many bands should appear in spectra is to do symmetry group theoretical analysis. It works well if you sample has not complex structure. The other way try to predict a number of bands use 2D correlation spectroscopy. Unfortunately, for all technics above require some additional information besides your spectra.
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