How to establish the HPV VLP purification platform from lab-scale to pilot-scale.
Hi Nataraja,
For the study of virus-like particles (VLP) suggest the paper DOI 10.1002/bit.22085 with protocol for expression and purification of each VLP.
Hello, We have a thermal cycler with the option to ramp down the temperature on % basis, however, I want to ramp down from 95 degrees Celsius to 25 degrees Celsius at 0.1 degrees Celsius per...
18 July 2022 6,220 0 View
Upon searching many literature many doubts regarding How to use CCA for downscaling of predictands. 1. How to input data into CCA? 2. How to do downscaling using CCA?
12 July 2017 9,755 2 View
How to estimate the dithiothreitol (DTT) concentration in purified protein (HPV antigen)?
20 December 2015 412 2 View
how to estimate Cetyl Trimethyl Ammonium Bromide concentration in pneumococcal purified polysaccharide .
11 September 2015 2,509 0 View
Can anyone tell me the mechanism of action of CTAB on the Polysaccharides Purification ?
18 May 2015 6,256 6 View
Weed Control
05 September 2012 5,523 7 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View