How to estimate the dithiothreitol (DTT) concentration in purified protein (HPV antigen)?

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Ramping down temperture in thermal cycler?

Hello, We have a thermal cycler with the option to ramp down the temperature on % basis, however, I want to ramp down from 95 degrees Celsius to 25 degrees Celsius at 0.1 degrees Celsius per...

18 July 2022 6,220 0 View

Can anyone explain how Canonical correlation analysis used for downscaling predictands

Upon searching many literature many doubts regarding How to use CCA for downscaling of predictands. 1. How to input data into CCA? 2. How to do downscaling using CCA?

12 July 2017 9,755 2 View

How to estimate the Cetyl Trimethyl Ammonium Bromide concentration in pneumococcal purified polysaccharide ?

how to estimate Cetyl Trimethyl Ammonium Bromide concentration in pneumococcal purified polysaccharide .

11 September 2015 2,509 0 View

Can anyone tell me the mechanism of action of CTAB on polysaccharide purification?

Can anyone tell me the mechanism of action of CTAB on the Polysaccharides Purification ?

18 May 2015 6,256 6 View

Why do we have to add "weed free check" treatment in weed control experiments ?

Weed Control

05 September 2012 5,523 7 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...

28 July 2024 6,515 1 View

What is effective targeted chaos?

Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...

28 July 2024 473 1 View

I want to get updates regarding international law, international trade law, international institutions and human rights etc?

Please send me updates regarding the above-mentioned areas in the question mark?

28 July 2024 5,197 3 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What is human-computer interaction (HCI)?

22 July 2024 10,056 2 View

Enquire about the calculation of percentage of reads mapped to the reference sequence?

Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...

20 July 2024 9,705 0 View