How to ensure that a chemical synthesized peptide has correct sequence?

More Mokhtar Nosrati's questions See All

How do we decide BSA percentage in our buffer solution?

Hi all, I am trying to test my polymer stability in biological medium. I know how to make 1% /5% etc of BSA in buffer but I am not sure how do we actually decide this percentage volume. Is there...

05 January 2021 649 2 View

Prevent contamination of solution in oven?

Hi all, I am trying to test stability of my electrode immersed in solution. I intent to keep them in oven for 3 weeks as to represent accelerate ageing. However, my worry is that the solution...

28 October 2020 8,351 2 View

Can graphene layers be coated by CVD on hydroxyapatite powders?

Is the hydroxyapatite decomposed during the CVD process?

29 February 2020 5,443 3 View

What is characteristic peak(s) in FTIR spectrum of crude plant extract?

Hello, I synthesized chitosan nanoparticles loaded with a plant extract. For confirming presence of the extract in the nanoparticles I did a FTIR analysis. Now my question is: What is...

24 January 2020 4,402 2 View

What do you suggest to me like books, articles to start working with isogeometric analysis?

I am looking to start working with the isogeometric method to analyze mechanical behavior such as beam, plate, shell and other structures of complex shapes. can you help me by giving me simple...

28 December 2019 2,729 5 View

How to measure fintech in banking sector?

Hi, I am currently pursuing my Phd study whereby my topic related to fintech and the effect towards green banking, and I am now in the process of understanding the LR about Fintech. Since Fintech...

08 December 2019 1,391 5 View

How to measure Moderator variable with SPSS?

Dear researchers, I want to learn how to measure moderator variable with SPSS. If you have any resources or videos i will appreciate to share it with me.

23 June 2019 9,504 3 View

Can anyone send to me a free download plagiarism checker program?

I found many plagiarism programs that allow only 150 words for checking. I search for a free download plagiarism checker program (not trial). Thanks

20 March 2019 5,708 6 View

Simple examples ,books and papers about The generalized differential quadrature method (GDQ) ?

I want to start working on the generalized differential quadrature (GDQ) method to solve the problem of the mechanical behavior of the beams, the plates and the shells. Dear colleagues , I...

13 November 2018 7,650 3 View

Which copper precursor if preferred for cu-nanoparticles synthesis in a green synthesis method?

Hi every one, I would like to synthesis of Cu Nanoparticles using plant extract, but in previous works different precursors have been used ( chloride and sulfate salts) , based on your experience...

17 October 2018 5,926 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the error of the Txy diagram?

Hi, I know the attached Txy diagram has an error but can't figure it out. Could you please look at the diagrams and explain it to me?

23 February 2021 4,239 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Andrei Blasko

Do peptide mapping (LC-MS).

Patrick Timmons

In addition to LC-MS mentioned above, you can deduce the peptide's sequence using a combination of TOCSY and NOESY NMR spectroscopy.

Erome Daniel Hankore

Hi Mokhtar,

I think LC-MS is the most efficient way. You can confirm the purity as well as get the sequence. It's more old school but there is also Edman degradation.