How to ensure that a chemical synthesized peptide has correct sequence?

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Patrick Timmons

In addition to LC-MS mentioned above, you can deduce the peptide's sequence using a combination of TOCSY and NOESY NMR spectroscopy.

Erome Daniel Hankore

Hi Mokhtar,

I think LC-MS is the most efficient way. You can confirm the purity as well as get the sequence. It's more old school but there is also Edman degradation.