How to eliminate PrimPol primer repeats in rolling circle amplification read processing?

Amanda van der Sijs @Amanda_Van_Der_Sijs

04 April 2019 0 8K Report

I'm amplifying DNA with REPLI-g, using PrimPol and phi29DNApol. In the process, PrimPol generates primers of about 7 to 9 nucleotides long. As far as my understanding goes, these primes will form hairpin repeats, as this region is amplified again. Does anyone have a good method or reference to locate these repeats and elimiate them in order to assemble the reads correctly?

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Science method

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