I want to use a fluorescence microscope to observe in situ the venom gland of a cleared spider using a 488 nm laser. Any recommendation on what dye to use that would be particularly attached to the venom sac or anything around it? Thank you
I might suggest trying Acridine Orange. A recent review of uses might give you some ideas of what it shows and how it might be used.
https://www.frontiersin.org/articles/10.3389/fonc.2019.00925/full
Acridine orange hydrochloride, Acridine orange zinc chloride or Acridine orange base? Upon searching the literature, there's little to no mention of this, the reagent generally being mentioned as simply Acridin Orange (AO). However, there is a big difference between them, AO hydrochloride being ( I think) the most suitable for cell permeability.
In the paper suggested by Robert John Wolff , indeed, it's mentioned AO (C17H19N3) which is AO base.
Acridine Orange is a good search term for basic information. A google search will turn up many articles, sources for purchase and basic information about its use. The Wikipedia article is a good place to begin if you want information about this stain: https://en.wikipedia.org/wiki/Acridine_orange
Cristian Emilian Pop .-. Have your tried the AO yet, or some other stain? How did it work?
Thank you all for your prompt and courteous answers. I'll try your suggestions after the holidays and will report back if it worked on the SPIM.
Robert John Wolff I used AO base to determine BPA in aqueous solution after Peijian et. al 2014 and it worked well
Cristian Emilian Pop .-. Thanks for the answer. I am interested in having someone (with lab & instrumentation) to see how BPA is transferred from the paper register receipts (heat printed). There are many questions about the transfer and absorbtion of BPA into the human body.
Robert John Wolff only gc-ms or more refined analysis can sort that out, I am currently using it to determine BPA in a known macrophyte used to epurate waste waters. I suppose that due to the minute amounts and the complexity of absorption, the transfer of BPA from heat print receipts to human is something challenging. It is known to be glucuronidated in humans via UDP-glucuronosyltransferase (UGT) to its glucuronidated form (BPA-Glu) but not too much is actually known for this more than half a century old chemical
Cristian Emilian Pop .-. Thanks for the answer. If you do want to study the pickup of BPA (external) on skin I have an experiment for you (or someone else in RG land) to conduct. One researcher here in my state was going to work on it, but an unexpected death cut that short. He did say the methodologies and accuracy were very problematic.
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