How to do reaction of amino acid on 2nd position of 2 hydroxy 1,4 naphthoquinone?

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Plant tissue culture: ammonium sulfate instead of ammonium nitrate? what concentration?

Plant tissue culture: ammonium sulfate instead of ammonium nitrate? what concentration? how to prepare stock 1 solution for MS media using ammonium sulfate?

07 May 2023 4,177 3 View

Can I use DEAE Sepharose in place of DEAE Cellulose?

I have been trying to purify Amyloid Beta peptide using ion exchange chromatography. In the paper I am referring to, they have used DEAE Cellulose in their protocol. I have been using Q-Sepharose...

15 January 2023 2,501 0 View

How to reduce noise in OCP and EIS study using Potentiostat?

Dear all, I have been doing trials for accessing corrosion properties of steel rebar in chloride environment using Potentiostat, wherein my working electrode is steel rebar, reference electrode is...

15 November 2022 8,797 0 View

Dear all I wanted to Ask is there are any traditional method for isolation of T Cells?

is there any traditional method to check T cell levels or can we isolate T-cells to check Th17 cell levels using specific markers? I am a little stuck here. Just to clarify I don't want to use...

20 September 2022 1,266 1 View

Protocol for induction of shooting from callus chickpea?

I get callus from chickpea genotype but i want to know which types of media and hormones concentration required for generation of shoot from callus

30 August 2022 637 0 View

Hi everyone. So I'm trying to locate nanoparticles inside plant tissues. Is there a technique I could use that can detect them without sectioning?

I need a rapid protocol for the detection of any spectral signature of nanoparticles inside plant tissues without necessarily sectioning the tissue. Is it possible?

28 August 2022 2,370 0 View

What is best protocol for estimation of proline, MDA content Catalase and SOD for plant leaves ?

02 June 2022 2,229 2 View

What is best method of surface sterilization of explant in tissue culture ? and how to minimize fungal contamination in tissue culture ?

what is best method of surface sterilization of explant in tissue culture ? and how to minimize fungal contamination in tissue culture ? Can I use Fungicide for surface sterilization of explant...

02 May 2022 8,709 4 View

How to use Case study method in Design Management research?

I'm conducting research to understand the design management practices in early stage start-ups. I 'm taking a action research and case study method as my approach. Would like to connect with...

14 February 2022 7,613 0 View

What type of plasticity model and its data for wood material should be input in ABAQUS software?

I am an M.Tech structural engineering student working on the project ' Numerical analysis of Kath-kuni architecture ( a common masonry typology ) in Himachal Pradesh region of India subjected to...

11 January 2022 1,154 3 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View