If you have the purified protein, you can run several lanes of it containing different amounts. Then when you do the Western blot, you can plot a standard curve of the signal for the purified protein. This can then be used to quantify the amount of the protein in the EV extracts.

Hi Sg MI,

Selecting an appropriate loading control for Western blotting of EV proteins is crucial for accurate quantification and normalization of protein expression levels. Common loading controls used in Western blotting for EV studies include proteins that are expected to be stable and present in relatively constant amounts in the EV samples.

Commonly used loading controls for Western blotting of EV proteins are

:

When selecting a loading control, it's essential to consider the specific type of EVs being studied (exosomes, microvesicles, etc.) and the proteins of interest. Loading controls should be chosen based on their stability and consistent presence in the EV samples.

I hope the info above will help.

Best,

Murat