What is a good marker to visualize sperm cell membrane with immunofluorescence?

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Malcolm Nobre

Hello

Try CD44.

Best Wishes.

Debadrita Pal

Try cell mask. It's like a dye that labels the cell membrane. (Something off this kind works good for me- https://www.thermofisher.com/order/catalog/product/C37608#/C37608)

So basically just put the cells in this dye and the membrane would get stained easily. Also these are available in different colors. And you could do live as well as fixed cell experiments with it.

John Griffin

A great list of plasma membrane stains can be found at: https://www.thermofisher.com/au/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html?gclid=EAIaIQobChMIhbLw0qC-8AIVRDVyCh0glgFpEAAYASAAEgIBgPD_BwE&ef_id=EAIaIQobChMIhbLw0qC-8AIVRDVyCh0glgFpEAAYASAAEgIBgPD_BwE:G:s&s_kwcid=AL!3652!3!355202100909!p!!g!!plasma%20membrane%20staining&cid=bid_pca_iva_r01_co_cp1359_pjt0000_bid00000_0se_gaw_nt_pur_con

Sg Ml

Thank you everyone!