I'm working of endophytes. I'm doing my extraction procedure with a little bit confusion. is that necessary that we have to do the primary extraction with ethyl acetate.
In most articles they suggested to do in ethyl acetate.
as i dont know what will be compound present in the microbe, can u tell me what should i do?
Tresha Dobson
The use of EtOAc is under the assumption that it is polar/non-polar enough to extract all metabolites (both non-polar n polar). Additionally caustic enough to break cell membranes. You can use other solvents if you so desire (chloroform, butanol) or just soak the cells in methanol etc with mechanical agitation.
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