Pls help! I would like to do IF staining with 2 antibodies on a metaphase spread. But I dont have a cytospin! How to do it without cytospin? And im using methanol:acetic acid fixation.
Thanks very much for your kind help Martin. But going through the dicentric assay it mentioned, I realised that it is using Hoechst and Giemsa which can be directly applied onto the cells. But I would like to use primary & secondary antibodies staining to detect centromeric proteins in the metaphase spread.