Linda Julian

6 Questions 12 Answers 0 Followers

Questions related from Linda Julian

MDR1 (ABCB1) western blotting?

Has anyone seen MDR1 bands below 75 kDa? I am using a monoclonal antibody and I am expecting a band at 170 kDa

16 May 2024 8,739 4 View

How can I dissolve my drug in 0.5% methylcellulose?

I am preparing a drug for oral gavage which needs to be dissolved in 0.5% methylcellulose. The drug powder remains as clump and refuses to go into solution. Any tips on this?

02 April 2024 4,937 3 View

How to do lentiviral transduction with two different vectors?

If I want to transduce cells with two lentiviral vectors, do I perform the transduction one after the other? Or do I collect the lentivirus from the different HEK plates and co-transduce my target...

08 November 2023 7,681 5 View

What is the optimal pH range for oral gavage solutions for mice?

I am planning to administer chemotherapeutic drugs to nude mice via the oral gavage route. What is the optimal pH range for the solution that can be given via oral gavage?

09 May 2023 8,957 1 View

Is 20% PEG 400 + 10% Cremophor + 70% PBS a viscous and cloudy solution?

I am making the following formulation for oral gavage in mice, 20% PEG 400 + 10% Cremophor + 70% PBS. The solution is viscous and cloudy. Is this normal?

14 April 2023 2,614 3 View

How much protein is required for Circular Dichroism?

What is the minimum protein concentration that is required to run a circular dichroism experiment and get a decent CD spectra?

01 September 2021 9,058 3 View