Dear all
I'm currently doing the cell morphology of different mutant strains I constructed and I used DAPI nucleic acid stain hoping to see what DNA distribution looks like in E.coli cells. I'm hoping to quantify the fluorescence intensity and its distribution across the different cells and showing it as a graph (sort of like making a model cell and show that what part of the cell has stronger fluorescence, like is it in the cell pole or mid cell.) But I'm having trouble making one since each of my strain I have taken more than one picture and I wished to combine different picture's data together to show the average. Does anyone know how to do it in MicrobeJ?
OR
if you ever analyze DAPI before, would you please share how you did the analysis with me?
Thank you so much!
If you have access to Imaris, you can draw lines through cells and get the intensity as a curve function of the cell's length. You can normalize all cells to a certain length (0 to 10 for example) and calculate the average.
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