No detection in Immunofluorescence Staining of control while no much difference in the both groups in western blot, why?

23 November 2020 8,529 3 View

For in silico analysis of a variant, howmany bioinformatic tools are required to give a sign in the favour of its pathogenecity?

Dear fellows, I am wondering about, how many bioinformatic tools are required to declare a variant as pathogenic and deleterious?

02 October 2020 6,580 3 View

Applying Plant extracts on cell line?

Has anybody tried applying plant extracts on MCF 7 cell lines after filtering them? will it (filtering) effect the antioxidant activity present in plants?

12 March 2020 5,258 3 View

How to find the thickness of graphene nanosheets by the AFM images?

i have seen many reports have used AFM images to find the thickness of graphene and graphene like structures. Is there any specific software or method to find the thickness of the Sheets.

14 January 2020 9,982 3 View

Why the BET Surface Area decreases with increasing carbonization Temperature?

I have prepared Carbon frameworks at 700 and 800(degree C) temperatures. The Surface area obtained for 700 (degree C) is higher than 800 (degree C).

01 January 2020 6,471 5 View

No title

02 June 2019 8,134 2 View

Given in 3D Ellipsoid semi major axis a=5 and b =4 ,c=3 . How can we find the distance D between two focal points (P0 and P2)?

Given in 3D Ellipsoid semi major axis a=5 (along axis) and semi minor axis b =4 ,c=3 (along y and z axis) . How can we find the distance D between two focal points (P0 and P2) ? Also how to find...

09 May 2019 8,093 4 View

How to fix the range of 't' (time) in matlab such that the delta function returns the impulse response for each dealy in matlab?

How to fix the range of 't' (time) in matlab such that the delta function returns the impulse response for each dealy in matlab. Can anyone help me in this regard ? I am having problem setting...

04 May 2019 8,787 3 View

How do we know either data is fixed effect or random effect model?

18 December 2018 1,443 4 View

How the bacteria can be differentiated visually in a mixed culture by adding aniline blue to MRS medium?

I am using mixed culture of LAB and Yeast for cereal fermentation.For enumeration of each bacterium in a mixed culture, I need to add anilin blue to the MRS medium but I didn't found how the...

23 July 2017 9,525 23 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View