Dear Pedro, If you are able to image the mobility of live cells through several hours, a chemotaxis chamber slide (e.g. Ibidi) would be one of the best options. In my experience Boyden chambers are not reliable enough for specific chemotaxis.

Thank you Teresa, very helpful! Best

Pedro

Dear Pedro,

I agree with Teresa. I used the Boyden chamber (and relative modification, such as the transwell insert) a lot and I never really liked it. The results are quite variable and the visualisation system not that reliable.

I personally never used the Ibidi chambers, but I use to make something similar connecting two 3.5 dishes with a glass capillar.

Another good alternative especially if you do not need high magnification, is the Dunn chamber; in this case the migration is from the center to the periphery so it is also relative easy to quantify the behaviour of a cell population. The down side is that if you need to go higher than 40x and use oil it become un-pratical.

Hope this help.

Good Luck!

Max