Hello, I have the problem that my griess reaction standard curve doesnt work because it always gets yellow at high concentrations and deep purple. Can you please help me.
I ran into this problem when I miscalculated my nitrite standard. Please check if you are really in the micromolar range (Griess assays typically measure 1-100 uM nitrite; reaction goes to orange/yellow, and eventually a brown precipitate, at ~100 mM nitrite) I am still trying to find information on what products are made in the reaction and how to safely dispose of them.