How to do 2D protein profiling of cocoa flowers ?

More Vijayakumar B Narayanapur's questions See All

How to change the protein chain names X into A, B, C, D after MD simulations in Gromacs?

I am running MD simulations for holoenzyme in Gromacs. The protein has four chains like A, B, C, D. After MD simulations, All the chains are showing as X chain in VMD and even in the PDB file....

12 November 2020 6,228 1 View

How to filter few markers from a gene set?

I am not good in bioinformatics. I have a gene set which has some 1000 genes which are highly correlating with aging. I want to select some highly significant genes from this geneset for further...

04 November 2020 5,602 5 View

Correlation plot between Gene of Interest and miRNA?

Please suggest portals to retrieve normalized-quantile expressions of micro-RNAs (in RPM).My aim is to construct a correlation plot between the gene of interest and microRNA. I searched in human...

28 October 2020 6,398 3 View

I am trying to do an article on domestic migration. Can anyone suggest me a research gap area where I can do my research on?

We have seen statistically that the domestic migration of workers are increasing every year. People are moving from rural to urban areas for better work and life. This migration has brought in...

03 March 2020 5,445 4 View

How will artificial intelligence help to develop MSMEs ?

Artificial Intelligence is progressive and has wider impact on various sectors. I would like to know how AI is impacting Micro, Small and Medium enterprises. The ways in which it is enacting for...

30 January 2020 7,367 3 View

What will be the perfect markers to differentiate Neural Stem cells from differentiated Neurons?

I've been working on differentiating Rat fetal Neural Stem cells into Neurons with a drug of interest. The Problem here for me is I have used various Neuronal markers like MAP2, TUJ1, NFM to...

15 October 2019 7,114 5 View

Autophagy: After how many days of fasting does autophagy usually start?

Hi, Could you please help me to explore after how many days of fasting does autophagy actually start in (1) Humans (2) Animal models and/or (3) cell lines, please? Thank you in advance

30 June 2019 8,778 5 View

Is there any standarad method or procedure for estimation of water productivity or water use efficiency of integrated farming system?

In integrated farming system (IFS), water applied for one component is also utilized in another component. So how we can compute the WUE and Water productivity of any IFS?

01 April 2019 7,555 2 View

Why the SOD1 mutant model expressing the mutant protein but not the native protein?

I am working with drug activity on familial ALS model cells. I am using mouse Neural stem cells which express the mutated human SOD1 protein. I did western blot with the protein extracted from the...

14 March 2019 511 3 View

Multiple data mining classifier

1. combining multiple data mining classifier improve the performance of technique? 2.If so ,how to hybrid different classifier together ? 3.which technique is used for linking?

01 August 2017 6,705 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View

Muayed Fadhil Abbas

First of all, you must have a good practice of the process of protein profile, then you could contact my published articles on the effect of salinity on protein profile in citrus and date palm on research gate.

Suresh K Malhotra

Please see following publication, if it can give you any clue. Cocoa EST database: Comprehensive database of Cocoa Expressed Sequence Tags (ESTs)

https://www.researchgate.net/publication/286439882_Cocoa_EST_database_Comprehensive_database_of_Cocoa_Expressed_Sequence_Tags_ESTs

Article Cocoa EST database: Comprehensive database of Cocoa Expresse...