Cell death is programmed and depends on the growth conditions under normal conditions. Cells usually die in different ways, and this can be important when choosing a therapeutic strategy against disease. However, how can one experimentally distinguish between two closely programmed forms of death, such as necroptosis from anoikis (death caused by cell detachment)?
It is really very important and interesting issue, as how to differentiate between anoikis and necrosis under experimental conditions. However, some basics is as follow:
Anoikis plays a fundamental role in preventing inappropriate translocation and attachment of cells, which can lead to abnormal growth in an ectopic environment. Anoikis is largely regulated by interaction with the ECM and nearby cells. On the other hand, necroptosis is a regulated necrosis mediated by death receptors. This form of necrosis works against pathogen-mediated infections, morphologically characterized by cell swelling followed by rupturing of plasma membrane. Necroptosis is an alternative mode of regulated cell death mimicking features of apoptosis and necrosis.
Dear Shail K Chaube, your answer is important for understanding the theoretical background. But what should be done experimentally in order to obtain reliable information on distinguishing between two different pathways leading to cell death ?
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