Hello everybody,
We are currently analyzing the induction of autophagy upon heart tissue samples, and I would like to know if there is a way to distinguish the non-selective autophagy than the selective one (particularly mitophagy).
Thank you all,
The following links might help.
Article Monitoring and Measuring Autophagy
Article Tools and techniques to measure mitophagy using fluorescence...
Article Monitoring Mitophagy in Mammalian Cells
you can analyze selective mitophagy by interaction/co-localization study of mitochondrial marker protein and the autophagy specific adaptor protein LC3II.
Look at the following
Article Ubiquitination and selective autophagy
Article Mechanisms of Selective Autophagy
Article Non-selective autophagy
If you need a detailed explanation, look at thoae references. An experiment that could demonstrate selective autophagy (if positive results, if negative you will still not know) is if degradation or autophagosome recruitment is abolished by KO or KD of mitophagy receptors e.g. NIX, BNIP3, OPTN, NDP53,
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