What is the scale recommended to measure human's baseline mood?

I am working on my Final Year Project, interested to look into emotional measurement of consumers in selected food product. One of the aspects in emotion measurement, I am interested to find out...

01 October 2019 1,241 3 View

How to remove the KOH from ethanolic etherification without using extraction method?

Etherification was carried out between aromatic -OH group and alkyl chloride in the presence of ethanolic KOH. The precursor is a bulky dye compound that consists of an aromatic -OH group and an...

23 May 2019 7,830 5 View

What software quickly collect full content from tens of thousands of social media post?

Hi, I am doing online ethnography, need to analyse the content meaning, narrative and theme of people's social media posts on a specific social event. The problem is: There are tens of...

05 May 2019 6,456 3 View

Any open-access to Hydrology Sandbox Experiment data?

I am working on numeric hydrology model deveolopment with Finite Element Method. I am thinking to test the model with the laboratory Hydrology Sandbox Experiment Data to validate the model. So I...

08 April 2019 2,330 2 View

Why the siRNA could reduce mRNA expression but not protein expression?

Recently, I want to knockdown the expression of one gene with siRNA. There are four siRNA sequence targeting the gene. They can silence target gene expression by at least 75% at the mRNA level,...

08 July 2018 6,916 5 View

Is it possible for a polymer to have optical band gap of 1.3 V and electrochemical band gap of 1.7 V?

Based on the reviews I read, most polymers got close value for optical and electrochemical band gap. I tried to calculate these band gap from UV-vis spectra and cyclic voltammetry. But the band...

01 January 2018 9,567 2 View

How to balance the bonding strength and contact resistance in the junctions and interfaces between different materials?

26 July 2017 8,771 4 View

Can I use cellulose Soxhlet thimble instead of glass fibre thimble for the Soxhlet extraction in polymerization?

I read that most previous studies used glass fibre thimble instead of the cellulose thimble. The solvents are methanol, acetone, hexane, toluene, dichloromethane, chloroform. If I use cellulose...

15 May 2017 397 2 View

Why the entire FTIR spectrum shifted to the left after dimerization?

18 February 2017 1,096 3 View

How can I get freshly powdered KOH from commercially bought KOH pallets?

The KOH absorb atmospheric moisture quite fast when I manually powdered it.

06 February 2017 862 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View