How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

How to tranfect PMA-treated THP-1 cells?

I differentiated THP-1 cells to macrophages using PMA(100ng/ml) and then used lentivirus to deliver shRNA to knockdown my target genes. Unfortunately, it was too difficult to be transfected...

16 August 2020 5,073 3 View

How to separate phospholipids from betaine lipids using silica chromatography?

I tried separating betaine lipid from phospholipid and galactolipid from my 2D TLC plate but to no avail. Betaine lipid was found to overlap with some galactolipids under the condition of:- 1D:...

25 March 2020 641 3 View

Except literature search, how to know a chemical compound belong to which family?

I am writing a review of the herbal plants which requires to elaborate the phytochemistry information. I just wonder if there is any easier way to know which chemical compound belongs to which...

24 September 2019 7,868 3 View

Can anyone recommend a self-efficacy scale for dietary behavior?

I searched a lot and find most self-efficacy scales are for diabetes or CVD or weight loss, etc. Even the DIET-SE is about low-fat and low-salt, I fail to find a scale only about 'general healthy...

01 July 2019 4,202 3 View

How to fabricate/make silica spin column for DNA/RNA extraction?

Dear Friends, Does anyone know how to fabricate/make silica spin column for DNA/RNA extraction? Any books, references and videos are welcome. Thanks in advance. GH Yue

27 June 2019 2,456 3 View

Is it enough to identify a novel miRNA only by RT-qPCR?

24 September 2018 3,275 4 View

Do you have information of extremely stable DNA?

Dear friends, I am looking some extremely stable DNA sequences. If you have some info on this, please let me know. Regards, Gen Hua

24 June 2018 591 2 View

For the Sterile filtration of Mabs, is it necessary to consider the relation between the filtration speed and PS20/PS80 retention effect?

Content: Fot the Process Characterization of sterile filtration of Mabs, we found out that at the beginning of the sterile filtration of Drug substance, there will be a small portion of protein...

15 May 2018 6,846 1 View

Any need to do unit root test for very short time series?

I read that the validity of unit root test becomes very small if the number of observations is small. In this case, it is very likely to have Type II error, say wrongly accepting the null...

08 January 2018 1,505 11 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

Why does sodium metabisulfite generate turbidity when mixing with sodium hydroxide?

When I tried to mix a sodium metabisulfite solution with sodium hydroxide solution, the mixture solution quickly became turbid. Before mixing both were clear solutions. Please help explain the...

03 February 2021 8,075 2 View

I want to extract engine oil from a soil sample,please how much amount of anhydrous sodium sulphate am i going to add to 10kg soil?

Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate

01 February 2021 9,509 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View