I am trying to dissolve PCNB (Penta Chloro Nitro Benzene) to add it to the PARPH media but it does not dissolve in alcohol, DMSO, or water. Can anyone suggest something?
Hi, You need to heat 95% ethanol in water bath upto 70 degree and try dissolving PCNB. This should give better result. I am following the same way
Thanks, I will follow the steps ASAP. Also I am trying to quantify Phytopthora from soil on PARPH media and having real difficulty keeping off the Mortierella. Do you have any suggestion. May be the soil sample I am working with has very low propagules, but every time I keep getting this fungus on the plate and hardly any phyt.
Hi, I dont have much experience with soil Phytophthora because I work with P. infestans which is mostly on leaf but I remember there are lot of literature on isolation from soil. i can have a look if you want , it should be somewhere in my external hard disk
Phytophthora is one of the pathogens that is very difficult to isolate. Initially I too had a lots of problem
Any suggestions is good help. Ya, I am mainly trying to isolate and quantify from soil. Thanks for giving a feedback, I am able to dissolve PCNB now.
Hi Dear Shima, may You kindly let me know what species do You work on? then I can help You more effectively. All the Best,
If Mortierella and Pythium are disturbing You, then add 25-30 mg Hymexazol. However, this compound is more or less disturbing agent against Ph. palmivora, Ph. cactorum, and Ph. lateralis. I offer a book: Detection and Isolation of Soil Fungi by Pierre Davet and Francis Rouxel. Best Wishes,
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