I have performed silica gel column chromatography of MeoH extract using CHCL3:MEOH upto 90%MeoH with minimal percent of Glacial acetic acid(GAA).I tried to dissolve the fractions in many ways like MeoH,Meoh with water,EtoH,EtoH with water,DMSO(100% to 5%),DMF,fraction eluted gradient ratio,water,CHCL3,GAA. but its not working in any means.