I would like to estimate estrogen and its metabolites using serum samples by LC-MS method. Kindly suggest me a standard protocol for estimation with literature evidence.
Hello.
If possible, you should use LC-MS/MS. A single MS will probably be difficult to validate the analysis.
https://academic.oup.com/jes/article/4/6/bvaa047/5823274
a couple of articles that answer your question:
Article Longitudinal monitoring of endogenous steroids in human seru...
Article Steroidomics for Highlighting Novel Serum Biomarkers of Test...
good luck!!!
Luca
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