I have recently synthesised some short (around 20mer) peptides containing between 2 and 4 cysteines but I can't seem to dissolve them. The peptides have a net positive charge but contain around 50% hydrophobic amino acids. I am using the peptides in a biological assay so the solvent cant be to harsh (TFA is out). I have synthesised lots of other peptides with similar sequences where the cysteine is omitted and they dissolve a lot better, so I am sure it is the cysteine that is causing the problem. Anyway, does anyone have any suggestions on the best way to dissolve them.