Martin Ashby

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Questions related from Martin Ashby

Peptide ribbon structure in TEM images?

I took some TEM images of a bacteria incubated with an antimicrobial peptide. The image shows lots of background structures that take two forms, one appears to be in the form of a ribbon. Is it...

13 January 2017 7,994 3 View

How to dissolve cysteine containing peptides?

I have recently synthesised some short (around 20mer) peptides containing between 2 and 4 cysteines but I can't seem to dissolve them. The peptides have a net positive charge but contain around...

06 August 2015 2,981 20 View

What are the different methods used for high throughput screening of synergistic relationships betwen antimicrobials in vitro?

Most studies utilise the checkerboard technique or time kill assays to investigate synergistic relationships but neither of these are particularly high throughput. Can anyone suggest a method for...

10 July 2015 515 4 View

Are there any in vitro conditions that can be used to temporarily or permanently reduce the permeability of a bacterial cell membrane to peptides?

I am hoping to find a method in which a change in the environmental conditions for a bacterial cell results in a significant decrease in the ability of a short amphipathic peptide to cross the...

16 January 2015 3,718 2 View