Hello. I would like to dissolve Amphotericin B for PO admition, so I have to use non toxic solvent. This drug is lipophilic and insoluble in water. I couldn't find online how to dissolve it. Hope anybody here can help me. Thank you in advance!
Dear Alina,
Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30–40 mg/ml) and in dimethylformamide (2–4 mg/ml). Aqueous solutions cannot be sterile filtered due to poor solubility.
Therefore, you have two choices: (1)-Dissolve it in buffers pH 2 or 11, (2)-dissolve it in DMSO (this is non-toxic solvent).
Hoping this will be helpful,
Rafik
Dear Rafik,
Thank you a lot for your answer. I've read DMSO MSDS and it is slightly toxic. I am wondering would it be safe to give it to animals?
Dear Alina,
It is used without consequences in concentrations up to 70%. Therefore you might use a mixture of buffer 11/DMSO: 50/50 or Buffer 2/DMSO: 50/50 or 70%DMSO and /30% one of the two buffers.
Rafik
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