Alina Kolpakova

4 Questions 6 Answers 0 Followers

Questions related from Alina Kolpakova

What commercial antibody would recognize mKate2 but not RFP or TdTomato?

I work with Drosophila samples. I tagged my protein of interest with mKate2 tag and want to immuno-stain against this tag. The problem is that I also have TdTomato expressed in my samples. I would...

12 January 2025 5,865 3 View

How do I calculate the expansion factor and the distortion between pre- and postexpansion 3D images?

Hello all I am performing expansion microscopy experiments and I need to calculate the expansion factor and the distortion during the expansion.My data is 3D. I tried to use Elastix software but...

11 January 2021 3,310 4 View

How to dissolve Amphotericin B using non toxic solvent?

Hello. I would like to dissolve Amphotericin B for PO admition, so I have to use non toxic solvent. This drug is lipophilic and insoluble in water. I couldn't find online how to dissolve it. Hope...

28 March 2016 5,716 3 View

How do I dilute Rhodamine 123 for live tissue mitochondria staining?

Hello I am working on Drosophila testis and would like to use Rhodamine 123 to visualize mitochondria in the live tissue samples. I've ordered Rhodamine 123 from Sigma and received a vile with...

05 August 2015 3,826 8 View