How to dissociate mycobacteria for CFU enumeration?

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I'm expanding MSC from umbilical cord with standard culture condition, however they are differentiating. Does anyone know what might be happening?

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How should I proceed to model a structure combining organic molecule whit metal nanoparticle, using Gaussian 9 software?

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Can someone suggest articles about the effect of the COVID 19 pandemic on nurses' mental health?

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23 January 2024 2,157 1 View

Someone could help me to solve a difficulty in establish the parameters for Km analysis (Michaelis Menten)?

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Can i use the protease inhibitors during cell membrane vesicle preparation ？?

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What is the appropriate filter size?

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Help with in vitro testing of candida biofilm susceptibility?

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Sunagar Raju

In general you can use the sonication.

If you know the biochemical composition of EPS you can treat them with biofilm dispersal agents like glycosidases, proteases.

Also try with common biofilm-matrix -degrading enzyme dispersin B or cetylpyridinium chloride.

Best

Ruchi Paroha

Sonication is best way to disaggregate mycobacterial cells, followed by passing through 26-31 guage needle syringe. You can also use glass beads for the declumping of mycobacteria.

Letícia Sayuri Murase

Sunagar Raju Ruchi Paroha Thanks for the reply!