How to differenciate Bronsted acid sites in "nests" from isolated ones?

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How to improve 260/230 ratio in rna extraction for PCR?

The 260/280 ratio is ok between 1.8 and 2.0 however we were unable to advance due to the 260/230 ratio

13 May 2024 7,643 3 View

Could you recommend methods for analyzing soil extracts from the rhizosphere for organic compounds, including secondary metabolites?

Additionally, could you suggest technical analytical instruments suitable for this purpose?

11 March 2024 7,579 2 View

Why the best blocking solution to blot?

We already used milk: 5% to 1 hour, 7% to 2 hour, 10% to 1h, 5% overnight, but, , in all blockages it still marks the entire membrane, every run, there is a large band with no specific mark HELP

20 February 2024 7,346 0 View

What is the best way to homogenize spinal cord for blot?

I'm going to use the RIPA buffer, but I don't know if I should use the sonicator or polytron homogenizer

30 January 2024 7,432 1 View

Error in autodock 4 + pyrx?

"ERROR! Too many atom types have been found: maximum is 14; we cannot continue" Does anyone know how to solve it?

27 January 2024 6,988 5 View

What is the best way to do a cytokine blot?

I am trying to do a western blot of cytokines (iL-10, IL-6) of muscle tissue, using a strip of lysis buffer, 50 ug of proteins and blocking in 5% skimmed milk, however no signal appears, any tips?

24 January 2024 7,021 2 View

Are there elucidated signaling pathways involved in the differentiation of memory T cell subsets?

We are aware that T cells can specialize into various memory cell types, such as stem cell memory (Tscm), central memory (Tcm), effector memory (Tem), and resident memory (Trm) subsets. However,...

22 November 2023 683 1 View

How can I confirm that my cell culture is pure?

How can I confirm that the cells isolated from the abdominal cavity of rats, following peritoneal lavage with dPBS and antibiotics, and subsequently centrifuged and plated, are indeed macrophages,...

22 November 2023 224 3 View

What is the best way to quantify astrocytes and microglia in tissue?

I need to quantify microglia and astrocytes through immunofluorescence, however I would like to know the best way to quantify, whether fluorescence intensity or manual counting or some other type

19 November 2023 212 3 View

How to calculate the boiling point in ORCA?

Can someone give me an orientation? I want to calculate properties including boiling of chemical species, melting points, and packing efficiency. Can I do this using ORCA software? If the answer...

26 July 2023 832 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View