How to develop renal cell carcinomamodel in C57BL/6J mice ?

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How to determine tannin content in fodder pellets?

22 July 2024 8,162 3 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,212 4 View

Want to authenticate aquatic plant species Duckweed? Two University botanist are unable to confirm, any confirmed place where it can be done in India?

06 June 2024 5,845 0 View

How do we quantify the flow behavior in a drying drop?

Is it possible to quantify the flow without adding any polymeric beads? I'm interested to know whether there's dominant capillary or Marangoni flow. Aby suggestions would really be helpful.

05 May 2024 2,233 5 View

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week. How to fix?

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week..Dose anyone know how to fix it ??

19 April 2024 1,111 2 View

Alphascreen versus TR-FRET?

I want to quantify low cAMP levels generated of a constitutively active GPCR also by different mutants of this receptor. I have tried the cAMP ELISA kit from Cayman chemicals with very...

14 April 2024 7,319 0 View

Has AI taking a big leap in Science and Engineering?

AI is coming fast, and we have to adapt.

29 March 2024 2,315 1 View

Bone material: What this minimum of maximum strain of Human cortical Bone before fracture?

What this minimum of maximum strain of Human cortical Bone before fracture. I saw lot of papers showing different values.

27 March 2024 4,255 1 View

Does the presence of degenerate nucleotides in consensus sequences affect the results of population genetic analysis?

I want to run STRUCTURE on a nuclear gene of an insect species. The sequences that I have received are showing the presence of degenerate nucleotides in the consensus sequences. Do I need to edit...

29 February 2024 648 1 View

Why binding energy in XPS shifted to lower side (decrease in the value) in composite with respect to base material?

In composite material, two elements of the base material show a positive shift in the binding energy, while the third element shows a negative shift in the binding energy. What are the reasons for...

11 February 2024 367 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,707 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View