Deeksha Pal

4 Questions 11 Answers 0 Followers

Questions related from Deeksha Pal

How to develop renal cell carcinomamodel in C57BL/6J mice ?

Hello Everyone I want to develop renal cell carcinoma in C57BL/6J mice preferably by chemical method. Kindly pour in some suggestions if any one have an experience in this field. Thanks

08 May 2021 9,861 3 View

In MTT assay after formation of formazon crystals.Should we remove the media before adding DMSO ?

15 May 2016 9,965 2 View

Is it necessary to starve the cells prior to any drug treatment?

I want to check effect of my drug U0126 on cellular proliferation (cell line A498). How can I decide the time of incubation with drug and after how much time I should proceed with proliferation assay?

15 June 2013 7,279 23 View

What should be the appropriate time of treatment to a cell line if we we want to check the effect on gene expression?

I want to check the effect of an inhibitor on the desired gene expression in my cell line. After how much time should I isolate the RNA and check the expression; after 6hour, 12hour, or 24 hour?

30 May 2013 8,435 6 View