How does a researcher ensure that the data that he/she is using has not created a skewed interpret the same public health data sources?

14 January 2020 9,966 3 View

Reporting secondary data results of SmartPls 3?

Hi, Can anyone guide that which results of Smartpls 3 need to be reported in data analysis section when we use secondary data? As we get many results like reliability, validity, quality criteria...

01 November 2018 6,917 2 View

Can anyone tell me that which one is better for running mediation and moderation: Process macro by Hayes or AMOS???

I read different articles but still cannot understand the idea.

10 June 2018 7,200 3 View

Dear I have problem related my Agrobacterium contamination. Before Colony PCR I have many small particles in my (ependorf, 50ml tubes also flask)?

there are so many small particles in all of the above. I have change my media and water. I am using EHA105 strain for transformation. the PCR results are good. Can I use the culture for...

23 April 2018 9,787 7 View

What are the problems in teaching and learning speaking skills at undergraduate level?

I want to know the current practices and coping strategies of teaching speaking skills as well.

17 June 2016 3,467 3 View

How to purify bacterial RNA (mRNA) from the infected blood of buffaloes, free of Eukaryotic RNA to run NGS for RNA sequencing.

I am struggling to purify bacterial RNA (mRNA) from the infected blood of buffaloes.My concerned is that my bacterial RNA must be free of Eukaryotic RNA from blood and need more RNA concentration...

06 March 2016 5,539 3 View

What are the different methods to prevent proteus swarming on any culture media?

Swarmming of proteus on the whole agar plate.

27 May 2012 2,386 34 View

Hi. I am looking for theories related to women empowerment . Can you please guide me?

I am trying to find women empowerment theories. Your work is related to women empowerment so, i thought i should ask you.

01 January 1970 9,429 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View