How to develop a scale in management or marketing?

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A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

Does the percentage of damage between the controls (negative and positive) differ much?

My negative control cells had some damage, they are neoplastic cells and my anaplastic cell had even more damage in the negative control. Ok, but my question is, is there much difference between...

27 May 2024 7,529 2 View

How can we accurately identify landslide seismic signals from low SNR raw data collected by seismic stations?

We know that only large-scale landslides can be detected by nearby seismic stations. In my research, I discovered that even landslides with volumes reaching millions or tens of millions of cubic...

23 May 2024 3,027 0 View

Why my CD (circular dichroism) graph for DNA look like a wave but not a peak at aound 260 nm?

I tested DNA structure (5uM dissolved in 50 mM Tris) using JASCO-1500 but found no peak at wavelength 220-350. The results look like a wave shape throughout the wavelength...

14 April 2024 4,003 2 View

The impact of sports experience on future leadership at work，How is it affected? has no meaning?

How leadership skills in sports teams reflect leadership in later work, and how leadership skills developed in sports can more effectively serve management at work

21 February 2024 1,186 4 View

Why do some primary antibodies perform well in isolated single-cell staining, but not in tissue sections?

Hi: I am performing immunofluorescence staining on 100 um 4%PFA fixed pancreas sections using our self-made polyclonal primary antibody. Tissue was gradient dehydrated in methanol diluted in 0.2%...

28 January 2024 867 3 View

After IP, the protein we targeted for the study was 30 KDa above original molecular weight, is this a true situation because of protein form change?

We used mouse cell line HEI-OC1 and checked OCTN1 expression by WB, then we did IP cell lysate with anti OCTN1 antibody and IB with OCTN1 antibody, surprising! The band is way above IB with WB (30...

04 January 2024 6,926 0 View

Why there is a big gap between statistics and z-average ?

17th refers to the experimental parameter code Material is mesoporous silica nanoparticles (MSN-NH2). Use deionized water as dispersant and ultrasonic vibration. The theoretical concentration is...

02 January 2024 4,160 3 View

Should level-1 predictors be included in a multilevel model to investigate the cross-level interaction effects between level-1 and level-2 predictors?

In our study, each participant rated several organizations randomly selected from a big pool on some attributes (denoted as) x1, x2, and x3 and an outcome y, and each organization has multiple...

14 November 2023 2,756 5 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How does one derive the standard deviation of a scale?

Dear all, I am working on analyzing data from a survey on student satisfaction. The survey contains items with a 7-point Likert response format that produce 12 scales related to different areas...

05 August 2024 2,141 4 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

Daniela Buzova

I guess that the following might be useful for you:

Shimp, T. A., & Sharma, S. (1987). Consumer ethnocentrism: Construction and validation of the CETSCALE. Journal of marketing research, 280-289.

Noor Ul Hadi

Kindly find the attachments for EFA and CFA.....

Article An Easy Approach to Exploratory Factor Analysis: Marketing P...

Bilal Saeed

Please find here a very good paper as a sample and guide for scale development.

Title: "Development of a multi-item Operational Excellence scale: Exploratory and confirmatory factor analysis".

Link is below

https://www.emerald.com/insight/content/doi/10.1108/TQM-10-2020-0227/full/html