I am working on a project where at some point need to measure the duration of G1 phase in live- non fixed cells. How can I do that? Is there any markers for this phase that I can fluorescently label them?
Thanks in advance.
I seek to understand the theoretical basis for selecting the optimal temperature for the thermal regeneration process of exhausted adsorbents, such as activated carbon loaded with dye molecules....
28 June 2024 1,867 3 View
I am using CTAB method (Naser M. Salameh et al., 2014) for fungal DNA isolation and purification. During reagents preparation I am not sure weather I should autoclave 2X CTAB and Wash buffer. So...
09 June 2024 8,470 2 View
How can we choose the weight matrix in the Convolutional Neural Network? And how this matrix is related to the kernels used in the algorithm?
01 June 2024 2,150 2 View
In SPSS 25, I have a categorical variable which I would like to display in a frequency table. However, one of my categories was not selected by any of my respondents. I can generate a frequency...
31 May 2024 2,076 3 View
I need a code of opensees python for 3d structure for IDA analysis please introduce a good source for this.
30 May 2024 8,926 3 View
Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...
26 May 2024 4,753 2 View
book or article
20 May 2024 2,488 3 View
I have a protein that is 117 amino acids long, with a 21 amino acid transmembrane domain at the C-terminus. I've cloned this protein into the pET28 expression vector in BL21, but am not seeing any...
20 May 2024 8,161 2 View
Please, if anyone has the GRTensorII (not GRTensorIII) algebra package for Maple send me. I tried to get it but I couldn't.
19 March 2024 8,067 0 View
require scientific analystis
15 February 2024 1,488 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View