Hadil Saleh

5 Questions 2 Answers 0 Followers

Questions related from Hadil Saleh

Do you seed cells at diffentent densities when performing timecourse experiment that run over 3 days?

what is the best way to approach time-course experiments that have long time internals, e.g. up to 3 days or even 6 days? I usually seed the same number of cells but cells in the 48hr and 72hr...

23 February 2021 2,556 0 View

How do you define DNA repair foci following ionisation radiation?

Hi everyone? what approaches have you guys been using to count and identity DNA repair foci follow gamma H2AX staining? Do irritated cells also form foci? do you measure the number of foci per...

21 May 2020 742 0 View

How to determine the duration of G1 phase in vivo?

I am working on a project where at some point need to measure the duration of G1 phase in live- non fixed cells. How can I do that? Is there any markers for this phase that I can fluorescently...

28 April 2020 7,918 0 View

Can anyone suggest an alternative method for the Cre-LoxP that is used for regulating gene expression?

I am working on a project and I was to restore the function of a specific mutated protein. I was advice to use the Cre-Lox recombination, however I was wondering if there are any other...

28 February 2020 6,345 1 View

How to study the stability of p53 in cell?

How are the levels of p53 monitored ? what techniques are used to study the stability of p53 during the cell cycle?

17 December 2019 7,423 4 View