We've try to use the substances like PE (polyethylene) and DEAE Dextran to prove the existence virus in monolayered cell, but the result is too confusing. Please tell me if you have the explanation about this.
PRRS, an arterivirus (RNA viruses) and we use Eagle MEM as medium growth with 10% of FBS. the incubation that we have been studied from many journals was approximately about 2-3 days. so we use it. I thought that we use the regular one.
can you give explanation about this?
Hello Ruben,
I understand that you are trying to trensfect Vero cells with some kind of plasmid using the DEAE Dextran method?
Vero Cells are not very easy to transfect. If you must use them try to use reagent of the Lipofectamin family. In any case I would try to optimize transfection first using a plasmid that expresses a reporter such as GFP before you start with your virus expresssion.
I hope this helps.
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