Please look at the following below links which may help you in your analysis:

https://pubs.acs.org/doi/10.1021/cm061596d

http://www.doiserbia.nb.rs/img/doi/0354-9836/2017/0354-98361500026K.pdf

http://www.biotechrep.ir/article_69202_e635cabd52ca76595f432e1abbdde2e8.pdf

Thanks

You can follow the kinetics of the CuO nanoparticles formation using UV -Vis spectroscopy on developing calibration curve.

You can use either UV-Vis spectroscopy or ICP.

You can analyze the Cu concentration by ICP and calculate CuO concentration. You can also use X-Ray Florescent (XRF) for analyzing the CuO in your solution.

You can go for X-ray photoelectron spectroscopy to examine the elemental composition . To characterize the optical properties of CuO, you can go for UV-vis absorption spectroscopy and to study the crystal structure, there is X-ray diffraction and Raman Spectroscopy.

Thank You.