How do you annotate figures to show significance?

30 January 2021 1,008 3 View

Can the benefits of resilience be linked to the types of resilience?

Hi All I am trying to obtain journal articles on the benefits on resilience. However, I was thinking - can the types of resilience (physical, emotional, psychological & community...

02 January 2021 7,409 6 View

In light of the pandemic, do you think early childhood curriculum must be changed accordingly to include topics related to the virus?

Curriculum Reform

13 December 2020 9,257 27 View

In light of the fact that schools are closed and student teachers cannot go out to be assessed on the field. How can we assess online?

Strategies for assessing practical work of student teachers.

26 November 2020 8,422 24 View

Does anyone have a questionnaire measuring Goleman's EI Model?

Hi Does anyone have a questionnaire measuring Goleman's EI Model? I want to use it on university academics. Many thanks

15 November 2020 2,839 3 View

What are the definitions of the 6 basic emotions (Paul Ekman)?

Hi All I am researching Paul Ekman's Basic Theory. Does anyone have any books or journal articles between the years 2016-present discussing the definitions of these 6 emotions: happiness,...

21 October 2020 5,317 8 View

What creative , interesting techniques do you use to keep tertiary education students fully interactive in an online session?

-Share your experiences and techniques

06 September 2020 1,770 5 View

How can peptides based on allele sequences be delivered to mice?

I am looking to measure allele-specific immune responses for specific antigens. After identifying the most prominent alleles in a population, I am looking to express them in mice and then measure...

03 September 2020 9,281 1 View

Missing values in R lme(): how to include observations with missings in the outcome variable?

Hello, I am struggling to understand how I best use the na.action options in R's lme function for a hierarchical linear model. I have a longitudinal data set with a two-level nested structure...

23 July 2020 2,327 4 View

What emotional intelligence theory is best for university academics?

Hi Please advise. If looking at the relationship between emotional intelligence and resilience in university academics - what would be the best EI theory to use? Considering we have ability...

09 June 2020 995 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View